Fig. 2

DDLPS cells reconstituted with miR-133a exhibit reduced cell growth in vitro. a Over expression of miR-133a validated using real time RT-PCR. Data are plotted as mean ± SEM for each miRNA performed in triplicate. *p < 0.05. b Real time quantitative PCR analysis was performed with primers against the CTGF gene. Primers to B2M were used to normalize the samples. Error bars represent standard error of mean for the replicate values. c Cell growth shown as measured by determining percent confluence from phase-contrast images normalized to plating density. DDLPS cell line 246 was transduced with lenti-miR-133a (red) or control (black). Two-way ANOVA was performed to determine statistical significance (p < 0.05). Data are shown as mean ± standard error of mean from three independent experiments. d Cell cycle progression analysis shown as distribution of cell phase at 15, 26, 33, 36, and 48 h time points. Data are representative of three independent experiments