Fig. 6

Cell apoptosis was promoted after transfection with CDA shRNA. Cell apoptosis rates in the shRNA-1 and shRNA-2 groups were higher than that in the CDA mimic group. a Cell apoptosis was detected by flow cytometry. The left lower quadrant of flow cytometry scatter plot represented healthy living cells (FITC−/PI−), the left upper quadrant represented the cells (FITC−/PI+) with mechanical damage, the right lower quadrant represented the cells (FITC+/PI−) in early apoptosis, and the right upper quadrant represented the cells (FITC+/PI+) in necrotic and late apoptotic stages. The apoptosis rate = percentage of cells in early apoptosis + percentage of cells in advanced apoptosis; b histogram analysis for the rates of early apoptosis (FITC+/PI−) and late apoptosis (FITC+/PI+) in each group; the data were expressed as mean ± standard deviation, n = 3, and One-Way ANOVA was used to analyzed the data. *Compared to the blank group, p < 0.05. CDA cytidine deaminase, FITC fluorescein isothiocyanate, PI propidium iodide