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Fig. 4 | Cancer Cell International

Fig. 4

From: Benzimidazoisoquinoline derivatives inhibit glioblastoma cell proliferation through down-regulating Raf/MEK/ERK and PI3K/AKT pathways

Fig. 4

Compound-1H induces apoptosis in human glioblastoma cells. U87 and LN229 cells were exposed to various concentrations of compound-1H for 48 h. a Cells treated with compound-1H were stained with annexin-V/PI. The Q4 (annexin-V−/PI−), Q3 (annexin-V+/PI−) and Q2 (annexin-V+/PI+) quadrants represent the populations of normal, early apoptotic and late apoptotic cells, respectively. b Percentages of surviving cells, early and late apoptotic cells are shown as the mean ± SD (n = 3). c Apoptotic nuclear morphological changes induced by compound-1H (0, 15, 30, 60 μmol/L) for 48 h were observed using Hoechst 33342 staining in U87 and LN229 cells. The arrowheads indicate apoptotic cells which exhibit highly condensed and fragmented nuclear morphologies. d Percentages of apoptotic cells are shown as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group

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