Fig. 2

Tamoxifen-resistant cell line is more sensitive than MCF7 to demethylation by 5-Aza-dC treatment. Scatter plots indicating genome-wide methylation differences between the Tamoxifen-resistant cell line, TMX2-28, and the parental line, MCF7 (a), between 96-h 5-Aza-dC-treated MCF7 and MCF7-Control (b), and between 96-h 5-Aza-dC-treated TMX2-28 and TMX2-28-Control (c). Each black dot on the plot represents a CpG site analyzed on the Human Methylation 450 BeadChip. Center red line represents equal average beta values in the two samples and outer red and green triangles indicate the number of CpGs with increased or decreased methylation relative to the cell line on the x-axis, respectively, based on a 1.8-fold change in average beta values