Fig. 6
From: TROP2 methylation and expression in tamoxifen-resistant breast cancer
TACSTD2 expression and proliferation and stable expression of TROP2 in TMX2-28 and knockdown of TROP2 in MCF7. (a) TACSTD2 mRNA expression determined by RT-qPCR run in technical triplicate for MCF7 (non-transfected), MCF7-Control, MCF7-TACSTD2-Knockdown, TMX2-28 (non-transfected), TMX2-28-Control and TMX2-28-TACSTD2 cell lines (**p < 0.001). b The MTS assay was used to quantify cell proliferation in MCF7-TACSTD2-Kd and TMX2-28-TACSTD2 cell lines (n = 16), represented as percent of control for each respective cell line (***p < 0.0001). c Expression of TROP2 determined by IHC for MCF7-Control-Clone and MCF7-TACSTD2-Knockdown-Clone. d Expression of TROP2 determined by IHC for TMX2-28-Control-Clone and TMX2-28-TACSTD2-Clone. Magnification = 200×