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Fig. 3 | Cancer Cell International

Fig. 3

From: MiR-98 modulates macrophage polarization and suppresses the effects of tumor-associated macrophages on promoting invasion and epithelial–mesenchymal transition of hepatocellular carcinoma

Fig. 3

HCC-conditioned TAMs promote proliferation, migration, invasion and EMT of SMMC7721 cells in vitro. SMMC7721 cells were incubated with culture medium of M0, M1, M2 and TAMs cells for 48 h. a MTT assay showed that TAMs promoted proliferation of SMMC7721 cells in vitro. b Wound healing assay demonstrated that TAMs promoted migration of SMMC7721 cells in vitro. c Transwell invasion assay showed that TAMs promoted invasion of SMMC7721 cells in vitro. Data are presented as the mean number of the migration and invasion cells per filed counted from five randomly selected fields under a microscope (×100 magnification). d Western blot analysis of EMT-related proteins showed that TAMs decreased the expression of E-cadherin but increased the protein levels of N-cadherin, fibronectin and vimentin, indicating that TAMs promoted EMT of SMMC7721 cells. Densitometric quantification was shown. **P < 0.01 vs. the control group

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Cancer Cell International

ISSN: 1475-2867

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