Fig. 3From: Activated gastric cancer-associated fibroblasts contribute to the malignant phenotype and 5-FU resistance via paracrine action in gastric cancerImmunofluorescent staining and semi-quantitative analysis of GCAFs. a Proteins for stromal and activation indication were detected by FITC-conjugated secondary antibodies, and the nuclei were stained blue with DAPI. Scale bar = 50 µm. b The expression levels were semi-quantitatively detected using western blot analysis. Vimentin and FAP were strongly expressed in all three strains of fibroblasts. α-SMA was relatively high-expressed in CAF-2922, and desmin was low-expressed in CAF-2916Back to article page