Fig. 1

Overexpression of Circ-ZEB1.33 in human tumor tissues and serum in HCC patients. a CircNet prediction of circ-ZEB1.33 regulation of miR-200a-3p and miR-140-3p targeting CDK6. The predicted regulatory links among cicRNA (purple dots), miRNA (yellow dots) and genes (blue dots) was listed, among which the regulatory axis of cir-ZEB1.33-miR-200a-3p/miR-1403p-CDK6 was emphasized using bold red line. b, c The transcription of circ-ZEB1.33 and miR-200a-3p in paired HCC tumor, adjacent tissues (n = 64) and normal liver tissues (n = 30) by real-time PCR. The relative-expression of circ-ZEB1.33 and miR-200a-3p were calculated using 2−ΔΔct methods compared to GAPDH and U6 respectively. d Linear correlation between miR-200a-3p and circ-ZEB1.33 in human HCC tumor tissues. e The existence of circ-ZEB1.33 in HCC and health control serum was detected by using real-time PCR. f Linear correlation between tumor and serum circ-ZEB1.33 in human HCC patients. The data were presented as mean ± SD, *P < 0.05, **P < 0.01, by Student’s t-test