Fig. 3

Circ-ZEB1.33 can sponge miR-200a-3p and attenuate the downregulation of CDK6. a The expression screen of circ-ZEB1.33 in different human HCC cell lines were performed by using real-time PCR. b A RNA pull-down assay was carried out by using probes for circ-ZEB1.33 and U6, the precipitation was amplified by using specific primer for 1: miR-200a-3p, 2: circ-ZEB1.33 (input), and 3: U6. c The prediction of potential miR-200a-3p binding to the 3′UTR of CDK6 was performed by using online tool TargetScan. d A luciferase reporter assay were performed for monitoring the CDK6 promoter activity regulated by WT and mutated 3′UTR of CDK6 in miR-200a-3p transfected Huh7 cells. The data were presented as mean ± SD, *P < 0.05, **P < 0.01, by Student’s t-test