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Fig. 4 | Cancer Cell International

Fig. 4

From: The lncRNA CCAT1 upregulates TGFβR1 via sponging miR-490-3p to promote TGFβ1-induced EMT of ovarian cancer cells

Fig. 4

miR-490-3p is essential for TGFβ1-induced EMT affected by CCAT1 knockdown via downregulation of TGFβR1. a Representative images of wound healing assay and quantification carried out in ovarian cancer cells (SKOV3 and CaOV3) transfected with sh-NC (scramble), sh-CCAT1 (CCAT1 knockdown), sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p mimics, sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p inhibitor. All cells were treated with 10 ng/ml TGFβ1. b Cell invasion assay and quantification showed invasiveness of ovarian cancer cells (SKOV3 and CaOV3) transfected with sh-NC (scramble), sh-CCAT1 (CCAT1 knockdown), sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p mimics, sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p inhibitor. All cells were treated with 10 ng/ml TGFβ1. c The mRNA level of TGFβR1 and EMT-associated markers (claudin, E-cadherin, N-cadherin, vimentin and MMP9) in ovarian cancer cells (SKOV3 and CaOV3) transfected with sh-NC (scramble), sh-CCAT1 (CCAT1 knockdown), sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p mimics, sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p inhibitor was detected by RT-qPCR. All cells were treated with 10 ng/ml TGFβ1. d Westernblot analysis showed the protein level of TGFβR1 and EMT-associated markers (claudin, E-cadherin, N-cadherin, vimentin and MMP9) in ovarian cancer cells (SKOV3 and CaOV3) transfected with sh-NC (scramble), sh-CCAT1 (CCAT1 knockdown), sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p mimics, sh-CCAT1 (CCAT1 knockdown) plus miR-490-3p inhibitor. All cells were treated with 10 ng/ml TGFβ1. All data were represented as mean ± SD from three biological replicates (*P < 0.05; **P < 0.01)

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