Fig. 2

Outgrowth of T98G cells under the influence of carnosine. T98G cells were seeded inside a cloning ring placed in a 12-well plate. Rings were removed after 4 h and cells were allowed to grow in the presence of 0, 10, 25, 50 and 75 mM carnosine. After 14 days, images were taken and the area covered by tumor cells was determined. The mean and standard deviation of the covered area from four independent wells for each concentration are presented in a. A statistical significant reduction was only seen at 50 mM carnosine (*) and at a concentration of 75 mM carnosine no tumor cells were left on the plates. In the lower part of the panel examples of images are presented that were used for data analysis (b without carnosine; c 10 mM; d 25 mM and e 50 mM carnosine). #2 presents the corresponding ImageJ processed pictures (Note: for presentation purposes the pictures shown have been vectorized and image enhanced using Corel Draw Graphic Suite 2017)