Fig. 4

TE-7 staining of T98G cells and fibroblasts in mono-, co- and in ring co-culture. The figure shows staining in mono cultures of fibroblasts (P0375) with DAPI (a), with a TE-7-specific antibody (e) and the merged image (i) and the corresponding images of staining of T98G cells (b, f, j). Images c, g and k were taken from a co-culture of both cells and d, h and f shows an image of a ring culture at low magnification. Fibroblasts appear with green staining in the cytoplasm and red nuclei, whereas T98G cells are presented by their yellow stained nuclei in the merged images. Scale bars a–c, e–g, i–k 30 µm, d, h, i 120 µm