Fig. 5

Cells migrating into the gap after removal of the cloning ring in a co-culture of T98G cells and fibroblasts. T98G cells (2500) were placed inside the cloning ring and fibroblasts (P0375; 50.000) outside of it. Migration of cells into the gap left after removal of the cloning ring 3, 4 and 11 days after ring removal with fibroblasts on the right side and T98G cells to the left as detected by bright field microscopy is presented. In the upper panel cultures were incubated in medium without carnosine and in the lower panel in medium containing 50 mM carnosine. Scale bar: 30 µm