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Fig. 3 | Cancer Cell International

Fig. 3

From: MicroRNA-524 promotes cell proliferation by down-regulating PTEN expression in osteosarcoma

Fig. 3

a TargetScan, miRDB and RNA22 are used to search for potential target genes of miR-524. After down-regulation of miR-524 expression in MG-63 (b) and 143B (c) cell lines, mRNA expression levels of each candidate target gene is analysed by qRT-PCR. d After down-regulation of miR-524 in MG-63 and 143B, PTEN protein expression was significantly increased. e miR-524 and PTEN 3′UTR binding sequences and PTEN 3′UTR mutant sequences. f, g Luciferase reporter assay used to verify direct binding between miR-524 and PTEN (***P < 0.001, **P < 0.01, *P < 0.05)

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