Fig. 7
Expression profile of autophagy and cell cycle arrest-related proteins in MDA-MB-231 mouse xenografts treated with AsIII and Tetra, alone or in combination. The experiment was carried out using human breast cancer nude mice implanted subcutaneously with 1 × 107 MDA-MB-231 cells. The administration of AsIII and Tetra, alone or in combination, and the preparation of tumor tissues and their protein samples were carried out as described under “Materials and methods”. Results are representatives of three independent experiments. The densitometry of protein bands was analyzed using a program, NIH ImageJ 1.52a. The values under each image represent the ratios between each key molecule and β-actin protein expression levels, which were further compared with those of vehicle-control group