Fig. 4

TAZ regulates mitochondrial fission. a, b Immunofluorescence staining of mitochondria. The average number of mitochondria was recorded. IL-2 treatment significantly activated mitochondrial fission, and this effect was further enhanced by TAZ deletion. c–f To quantify mitochondrial fission, the expression of mitochondrial fission-related proteins was analyzed. g Capsase-3 activity was measured in HepG2 cell transfected with TAZ siRNA in the presence of IL-2. To inhibit mitochondrial fission, Mdivi-1 was added to IL-2-treated cells. h–i PI staining was used to observe apoptotic cells. *P < 0.05 vs. control (Ctrl) group; ^#P < 0.05 vs. IL-2 group; ^@P < 0.05 vs. IL-2 + si-TAZ group