Fig. 5

TAZ-triggered mitochondrial fission causes mitochondrial dysfunction. a, b Cellular ROS production was measured by flow cytometry in HepG2 cell treated with TAZ siRNA in the presence of IL-2. Mdivi-1, an inhibitor of mitochondrial fission, was added to TAZ-depleted cells. c–e Antioxidant concentrations were measured by ELISA. f–g Immunofluorescence was used to analyze cyt-c leakage into the nucleus. h Caspase-9 activity was measured in response to TAZ deletion or mitochondrial fission inhibition. *P < 0.05 vs. control (Ctrl) group; ^#P < 0.05 vs. IL-2 group; ^@P < 0.05 vs. IL-2 + si-TAZ group