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Fig. 1 | Cancer Cell International

Fig. 1

From: Tumor-associated macrophages derived CCL18 promotes metastasis in squamous cell carcinoma of the head and neck

Fig. 1

In vitro polarization of THP-1 cells into M2-like TAMs. THP-1 monocytes were treated with the combination of PMA, rhIL-4 and rhIL-13. a mRNA expression of M2 macrophages markers (CD206, CCL18, IL-10 and CCL18) was quantified by qRT-PCR. b Cell surface proteins of CD206 and CD163 were analyzed by flow cytometry. c CCL18 and IL-10 secretion in culture medium was measured by ELISA. Results are shown as mean ± SD. **P <0.01, ***P < 0.001, ****P < 0.0001

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