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Fig. 3 | Cancer Cell International

Fig. 3

From: Tumor-associated macrophages derived CCL18 promotes metastasis in squamous cell carcinoma of the head and neck

Fig. 3

M2-like TAMs induce EMT and stemness in SCCHN in vitro. Tu686 cells cultured with M2 CM or M0 CM for 2 days. a Representative morphological images (original magnification 100×). b, c EMT proteins were examined and semi-quantified by western blotting. Similar with b and c, proteins (d and e) of EMT transcription factors Slug and Snail were also measured. f, g Images of tumorspheres and their quantification were shown. h mRNA expression of CSCs markers CD133, CCL44 and ALDH1 was quantified by qPCR. i Proteins of CD133 or/and CD44 were analyzed by flow cytometry. Results are shown as mean ± SD. *P < 0.05, **P <0.01, ***P < 0.001, ****P < 0.0001

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