Table 4 List of downregulated proteins in the Dox- and Sul-treated MDA-MB-468 cells
From: Sulbactam-enhanced cytotoxicity of doxorubicin in breast cancer cells
Protein name | Abbreviation | UniProt ID | Mass (Da) | pI | Spectrum count | Dox + sul/Dox | p value | Biological process | |
|---|---|---|---|---|---|---|---|---|---|
Dox | Dox + Sul | Folda | |||||||
60S ribosomal protein L4 | RPL4 | P36578 | 47,566.1 | 11.1 | 4.53 | 1.96 | − 2.31 | 2.25E−02 | Translation |
60S ribosomal protein L17 | RPL17 | J3QLC8 | 20,246.8 | 9.5 | 1.84 | 0.27 | − 6.76 | 4.24E−02 | Translation |
60S ribosomal protein L24 | RPL24 | C9JXB8 | 14,368.8 | 11.3 | 0.56 | 0.00 | − 100.00 | 1.30E−04 | Translation |
60S ribosomal protein L27a | RPL27A | P46776 | 16,430.2 | 11.0 | 3.20 | 1.47 | − 2.17 | 1.42E−02 | Translation |
60S ribosomal protein L37a | RPL37A | P61513 | 10,275.3 | 9.5 | 1.39 | 0.00 | − 100.00 | 4.08E−03 | Translation |
40S ribosomal protein S3a | RPS3A | D6RAT0 | 25,887.1 | 9.5 | 7.51 | 0.00 | − 100.00 | 4.01E−03 | Translation |
Eukaryotic translation initiation factor 1A, Y-chromosomal | EIF1AY | O14602 | 16,442.4 | 4.6 | 0.65 | 0.00 | − 100.00 | 1.43E−03 | Translation |
Eukaryotic translation initiation factor 1A, X-chromosomal | EIF1AX | P47813 | 16,460.4 | 4.6 | 0.65 | 0.00 | − 100.00 | 1.43E−03 | Translation |
Eukaryotic translation initiation factor 4 gamma 1 | EIF4G1 | B2RU10 | 176,207.3 | 5.4 | 1.12 | 0.00 | − 100.00 | 3.18E−02 | Translation |
Eukaryotic translation initiation factor 3 subunit J | EIF3 J | O75822 | 29,062.4 | 3.7 | 1.38 | 0.27 | − 5.10 | 1.16E−02 | Translation |
Eukaryotic translation initiation factor 6 | EIF6 | P56537 | 26,599.2 | 3.7 | 0.70 | 0.00 | − 100.00 | 2.17E−02 | Translation |
Nascent polypeptide-associated complex subunit alpha | NANA2 | Q13765 | 23,383.9 | 4.5 | 1.57 | 0.00 | − 100.00 | 1.47E−03 | Translation |
Nascent polypeptide-associated complex subunit alpha, muscle-specific form | NANA | F8VZJ2 | 15,016.0 | 4.9 | 1.57 | 0.00 | − 100.00 | 1.47E−03 | Translation |
Eukaryotic translation elongation factor 1 beta 2 | EEF1B2 | A4D1M6 | 24,891.0 | 3.7 | 1.26 | 0.00 | − 100.00 | 7.44E−03 | Translation |
Heterogeneous nuclear ribonucleoprotein D0 | HNRNPD | Q14103 | 38,434.2 | 7.6 | 4.25 | 2.39 | − 1.78 | 2.07E−02 | Translation |
MAP kinase-interacting serine/threonine-protein kinase 1 | MKNK1 | E9PMF1 | 12,586.3 | 9.5 | 0.84 | 0.00 | − 100.00 | 8.80E−07 | Translation |
Heterogeneous nuclear ribonucleoproteins C1/C2 | HNPNPC | G3V2Q1 | 33,570.9 | 5.0 | 3.50 | 0.81 | − 4.30 | 2.30E−02 | Translation |
KH domain-containing, RNA-binding, signal transduction-associated protein 1 | KHDRBS1 | Q07666 | 48,227.3 | 8.7 | 0.97 | 0.00 | − 100.00 | 1.21E−02 | Regulation of transcription |
High mobility group protein HMG-I/HMG-Y | HMGA1 | P17096 | 11,544.8 | 10.3 | 1.53 | 0.70 | − 2.19 | 2.87E−02 | Regulation of transcription |
cDNA FLJ54188, moderately similar to High mobility group protein HMG-I/HMG-Y | HMGA1 | B4DWA0 | 34,301.4 | 10.4 | 1.53 | 0.70 | − 2.19 | 2.87E−02 | Regulation of transcription |
Serrate RNA effector molecule homolog | SRRT | Q9BXP5 | 100,666.7 | 7.2 | 0.97 | 0.00 | − 100.00 | 7.88E−03 | Regulation of transcription |
Protein SIX6OS1 | C14orf39 | Q8N1H7 | 68,166.0 | 5.4 | 0.56 | 0.00 | − 100.00 | 1.30E−04 | Regulation of transcription |
Heterogeneous nuclear ribonucleoprotein D-like | HNRPDL | O14979 | 46,437.5 | 9.6 | 2.23 | 0.00 | − 100.00 | 9.63E−03 | Regulation of transcription |
Zinc finger and BTB domain-containing protein 14 | ZFP161 | O43829 | 50,956.5 | 5.4 | 0.74 | 0.00 | − 100.00 | 9.26E−03 | Regulation of transcription |
Golgin-45 | BLZF1 | Q9H2G9 | 44,910.4 | 9.1 | 0.70 | 0.00 | − 100.00 | 1.68E−02 | Regulation of transcription |
Zinc finger protein neuro-d4 | DPF1 | E9PDV3 | 45,285.6 | 7.2 | 0.56 | 0.00 | − 100.00 | 1.30E−04 | Regulation of transcription |
Histone cluster 1, H1e | HIST1H1E | Q4VB24 | 21,893.3 | 9.5 | 4.44 | 0.00 | − 100.00 | 1.25E−02 | Regulation of transcription |
Serine/arginine-rich splicing factor 10 | SRSF10 | O75494 | 31,300.5 | 11.2 | 0.56 | 0.00 | − 100.00 | 1.30E−04 | RNA processing |
Heterogeneous nuclear ribonucleoprotein Q | SYNCRIP | O60506 | 69,471.4 | 8.7 | 5.98 | 1.95 | − 3.07 | 3.52E−02 | RNA processing |
Transformer-2 protein homolog alpha | TRA2A | Q13595 | 32,688.6 | 11.2 | 1.11 | 0.18 | − 6.02 | 3.23E−02 | RNA processing |
Multidrug resistance protein 1 | ABCB1 | P08183 | 141,479.1 | 9.1 | 3.47 | 0.84 | − 4.13 | 5.08E−04 | Transporters |
ATP-binding cassette sub-family G member 2 | ABCG2 | Q9UNQ0 | 72,314.0 | 8.9 | 1.66 | 0.36 | − 4.56 | 1.05E−03 | Transporters |
ATP-binding cassette sub-family A member 8 | ABCA8 | O94911 | 179,245.9 | 9.1 | 0.56 | 0.00 | − 100.00 | 8.80E−07 | Transporters |
Sodium/potassium-transporting ATPase subunit alpha-4 | ATP1A4 | E9PRA5 | 57,244.4 | 9.1 | 0.56 | 0.00 | − 100.00 | 1.30E−04 | Transporters |
Syntaxin-8 | STX8 | Q9UNK0 | 26,906.8 | 3.7 | 0.56 | 0.00 | − 100.00 | 8.80E−07 | Transporters |
Wiskott-Aldrich syndrome protein family member 1 | WASF1 | Q92558 | 61,652.4 | 5.4 | 0.56 | 0.00 | − 100.00 | 1.30E−04 | Cytoskeleton organization |
Actin-related protein 2/3 complex subunit 2 | ARPC2 | O15144 | 34,333.1 | 9.1 | 1.23 | 0.00 | − 100.00 | 5.68E−03 | Cytoskeleton organization |
Actin-related protein 2/3 complex subunit 3 | ARPC3 | O15145 | 20,415.5 | 8.8 | 0.97 | 0.00 | − 100.00 | 7.88E−03 | Cytoskeleton organization |
Ras GTPase-activating-like protein IQGAP1 | IQGAP1 | P46940 | 189,120.8 | 6.1 | 1.25 | 0.00 | − 100.00 | 4.32E−04 | Cytoskeleton organization |
Myosin light chain 6B | MYL6B | P14649 | 22764.1 | 6.3 | 2.23 | 0.00 | − 100.00 | 8.80E−07 | Cytoskeleton organization |
TBC1 domain family member 31 | WDR67 | Q96DN5 | 124189.8 | 9.1 | 1.11 | 0.00 | − 100.00 | 9.75E−04 | Cytoskeleton organization |
Prelamin-A/C | LMNA | Q5TCI8 | 55762.4 | 6.6 | 10.73 | 0.28 | − 37.73 | 1.74E−02 | Cytoskeleton organization |
Lamin A/C | LMNA | W8QEH3 | 65116.9 | 9.1 | 11.69 | 0.00 | − 100.00 | 8.86E−03 | Cytoskeleton organization |
Calumenin | CALU | O43852 | 34961.1 | 4.5 | 1.66 | 0.36 | − 4.60 | 2.73E−02 | Cytoskeleton organization |
Lamina-associated polypeptide 2, isoforms beta/gamma | TMPO | P42167 | 50670.3 | 9.5 | 1.85 | 0.74 | − 2.49 | 2.75E−02 | Cytoskeleton organization |
Kinesin-like protein | KIF15 | A0A087X0P0 | 312105.2 | 5.5 | 2.19 | 0.00 | − 100.00 | 6.76E−06 | Cytoskeleton organization |
DnaJ homolog subfamily A member 1 | DNAJA1 | P31689 | 44868.4 | 7.2 | 1.26 | 0.36 | − 3.44 | 2.12E−02 | Protein folding |
T-complex protein 1 subunit epsilon | CCT5 | P48643 | 59539.8 | 5.4 | 3.47 | 0.41 | − 8.39 | 9.26E−03 | Protein folding |
T-complex protein 1 subunit beta | CCT2 | P78371 | 57357.0 | 6.0 | 1.11 | 0.00 | − 100.00 | 1.30E−04 | Protein folding |
Cysteine and histidine-rich domain-containing protein 1 | CHORDC1 | Q9UHD1 | 37489.9 | 7.2 | 0.55 | 0.00 | − 100.00 | 6.76E−06 | Protein folding |
CDC37 protein | CDC37 | Q6FG59 | 44453.5 | 3.7 | 1.78 | 0.41 | − 4.38 | 4.29E−02 | Protein folding |
26S proteasome non-ATPase regulatory subunit 7 | PSMD7 | P51665 | 37025.4 | 6.3 | 0.69 | 0.00 | − 100.00 | 1.95E−02 | Protein catabolic process |
Proteasome subunit beta type-3 | PSMB3 | P49720 | 22949.0 | 9.1 | 0.56 | 0.00 | − 100.00 | 1.30E−04 | Protein catabolic process |
Proteasome subunit alpha type-4 | PSMA4 | P25789 | 29483.8 | 7.6 | 0.65 | 0.00 | − 100.00 | 1.01E−03 | Protein catabolic process |
Ubiquitin carboxyl-terminal hydrolase 43 | USP43 | Q70EL4 | 122809.5 | 9.5 | 0.83 | 0.00 | − 100.00 | 4.42E−02 | Protein catabolic process |
Enolase-like protein ENO4 | ENO4 | J3KNX1 | 68464.9 | 6.3 | 1.11 | 0.00 | − 100.00 | 5.54E−03 | Carbohydrate metabolism |
PCK2 protein | PCK2 | Q6IB91 | 70697.2 | 7.2 | 0.70 | 0.00 | − 100.00 | 2.17E−02 | Carbohydrate metabolism |
Fructose-bisphosphate aldolase | ALDOC | A8MVZ9 | 36295.3 | 7.6 | 1.53 | 0.00 | − 100.00 | 2.78E−03 | Carbohydrate metabolism |
Cytochrome c oxidase subunit 5A, mitochondrial | COX5A | H3BNX8 | 17234.9 | 7.2 | 1.24 | 0.00 | − 100.00 | 4.42E−02 | Mitochondrial metabolic process |
Cytochrome b5 type B | CYB5B | J3KNF8 | 16694.6 | 6.3 | 0.82 | 0.00 | − 100.00 | 4.69E−02 | Mitochondrial metabolic process |
Cytochrome b-c1 complex subunit 1, mitochondrial | UQCRC1 | P31930 | 52646.0 | 7.2 | 0.56 | 0.00 | − 100.00 | 1.30E−04 | Mitochondrial metabolic process |
MICOS complex subunit MIC19 | CHCHD3 | Q9NX63 | 26152.4 | 9.1 | 0.70 | 0.00 | − 100.00 | 1.68E−02 | Mitochondrial metabolic process |
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial | PCK2 | Q16822 | 70730.2 | 7.2 | 0.70 | 0.00 | − 100.00 | 2.17E−02 | Mitochondrial metabolic process |
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial | DLST | P36957 | 48755.5 | 9.5 | 0.56 | 0.00 | − 100.00 | 1.30E−04 | Mitochondrial metabolic process |
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial | PDHA1 | P08559 | 43295.8 | 9.1 | 0.56 | 0.00 | − 100.00 | 1.30E−04 | Mitochondrial metabolic process |
Apoptosis inhibitor 5 | API5 | Q9BZZ5 | 59004.7 | 9.1 | 1.21 | 0.27 | − 4.44 | 4.79E−02 | Negative regulation of apoptotic process |
Epidermal growth factor receptor | EGFR | A9CB80 | 132022.7 | 6.2 | 6.03 | 0.54 | − 11.12 | 5.65E−03 | Signal transduction |
A-kinase anchor protein 9 | AKAP9 | Q99996 | 453668.7 | 3.7 | 0.84 | 0.00 | − 100.00 | 7.75E−05 | Signal transduction |
Rho GDP-dissociation inhibitor 1 | ARHGDIA | J3KS60 | 9944.0 | 4.2 | 0.65 | 0.00 | − 100.00 | 5.33E−03 | Signal transduction |
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit | PPP1CA | P62136 | 37512.2 | 7.2 | 1.81 | 0.00 | − 100.00 | 4.26E−03 | Signal transduction |