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Fig. 4 | Cancer Cell International

Fig. 4

From: Levetiracetam enhances the temozolomide effect on glioblastoma stem cell proliferation and apoptosis

Fig. 4

LEV effect on MGMT expression in GCSCs. GCSCs derived from patient #4 were treated with TMZ (250 µM), LEV (40 μg/mL) and with a combination of TMZ and LEV for 48 h. a, c Western blot analysis of MGMT and PCNA expression levels; representative immunoblots are shown. b, d Densitometric analysis of MGMT and PCNA expression levels (normalized to β-actin levels) of three independent experiments. Statistical analysis was performed using Student’s t-test. P value < 0.05 was considered statistically significant. e Confocal microscopy micrographs of GCSCs from patient #4 showing MGMT expression. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Original magnification: ×400

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