Fig. 3

Confirmation of B7-H6 knockdown efficiency in the HCC cell lines HepG2 and SMMC-7721. a, b Confirmation of the efficiency of infection of the HCC cell lines HepG2 and SMMC-7721 with recombinant lentiviral vector harboring B7-H6 shRNA by the analysis of GFP expression using fluorescence microscopy. c, d Validation of the decreased B7-H6 expression at the mRNA level after knockdown in HepG2 and SMMC-7721 cells using real-time RT-PCR analysis (P < 0.01 and P < 0.05, respectively). e Detection of B7-H6 expression at the protein level in the LV-NC and LV-B7-H6-shRNA groups of both HepG2 and SMMC-7721 cells using Western blotting. f Statistical analysis showed that B7-H6 expression at the protein level was significantly decreased in the LV-B7-H6-shRNA group compared with that in the LV-NC group in both HepG2 and SMMC-7721 cells (both P < 0.01)