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Fig. 4 | Cancer Cell International

Fig. 4

From: Expression of B7-H6 expression in human hepatocellular carcinoma and its clinical significance

Fig. 4

Contribution of B7-H6 knockdown to cell proliferation, migration, invasion and cell cycle in HCC cell lines. a The CCK-8 assay was used to examine the effects of B7-H6 knockdown on the cell proliferation rate in vitro in the human HCC cell lines HepG2 and SMMC-7721. In HepG2 cells, the proliferation rate of the LV-B7-H6-shRNA group was significantly lower than that of the LV-NC group at 48 h and 72 h (P < 0.05 and P < 0.01, respectively). In SMMC-7721 cells, the proliferation rate of the LV-B7-H6-shRNA group was significantly lower than that of the LV-NC group at 48 h and 72 h (both P < 0.05). b A wound healing assay was performed to examine the contribution of B7-H6 knockdown to the migration of HCC cells. In HepG2 cells, the cell-free area of the LV-B7-H6-shRNA group was significantly wider than that of the LV-NC group at 24 h and 36 h (P < 0.05 and P < 0.01 respectively) after the scratch was made on the cell monolayer, and in SMMC-7721 cells, the cell-free area of the LV-B7-H6-shRNA group was significantly wider than that of the LV-NC group at 36 h (P < 0.01) after the scratch was made on the cell monolayer. c The Transwell invasion assay indicated that in both HepG2 and SMMC-7721 cells, the number of crystal violet-stained cells was significantly decreased in the LV-B7-H6-shRNA group compared with that in the LV-NC group (at 24 h, P < 0.01 and P < 0.001 respectively, and at 36 h, both P < 0.001). d The flow cytometric analysis showed that in both HepG2 and SMMC-7721 cells, the LV-B7-H6-shRNA group displayed an increased percentage of cells in the G1 phase and a decreased percentage of cells in the G2/M phases compared with the LV-NC group

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