Fig. 2

Over-expression of miR-384 inhibited the proliferation of CRC cells in vitro and in vivo. a Over-expression of miR-384 in SW480 and HCT116 CRC cells verified by real-time PCR. b–g The proliferative ability of the indicated cells detected by MTT assays, colony formation assays and soft agar assays. Only cell colonies containing more than 50 cells were counted. Error bars represent mean ± SD from 3 independent experiments. **p < 0.01. h SW480/miR-384 and SW480/Vector cells were injected into the hind limbs of nude mice (n = 6). The tumor volume data were presented as the mean ± SD. i, j Histopathological analyses of xenograft tumours. The tumor sections were stained with H&E or subjected to IHC staining using an antibody against Ki-67. Error bars represent mean ± SD from three independent experiments. **p < 0.01