Fig. 3

Inhibition of endogenous miR-384 promoted the proliferation of CRC cells in vitro and in vivo. a Expression of miR-384 in LOVO and SW620 CRC cells transfected with inhibitor or their paired negative control lentiviral vector was detected by real-time PCR. b–g The proliferative ability of the indicated cells detected by MTT assays, colony formation assays and soft agar assays. Only cell colonies containing more than 50 cells were counted. Error bars represent mean ± SD from 3 independent experiments. **p < 0.01. e LOVO/miR-384-in and LOVO/NC cells were injected into the hind limbs of nude mice (n = 6). The tumor volume data were presented as the mean ± SD. i, j Histopathological analyses of xenograft tumours. The tumor sections were stained with H&E or subjected to IHC staining using an antibody against Ki-67. Error bars represent mean ± SD from three independent experiments. **p < 0.01