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Fig. 1 | Cancer Cell International

Fig. 1

From: SH2B1 promotes NSCLC cell proliferation through PI3K/Akt/mTOR signaling cascade

Fig. 1

Functional significance of SH2B1 in NSCLC cells in vitro. a, b FACS analysis with annexin V-PE and 7-AAD was used to evaluate the apoptosis. The FACS-apoptosis staining (a) showed an increased percentage of cell apoptosis in SH2B1 knockdown A549 cells (shSH2B1) compared with control cells (shCtrl) (11.55 ± 0.68% vs 6.45 ± 0.56%, P = 0.0007, n = 3). b A decreased percentage of cell apoptosis in SH2B1 overexpression H1299 cells compared with control cells (vector) (10.91 ± 0.30% vs 6.22 ± 0.20%, P = 0.0002, n = 3). Results are representative of three independent experiments. c, d SH2B1 regulated the c-myc and cleaved casp-3 in A549 and H1299 cells as confirmed by qRT-PCR and western blotting analysis, mean ± SD, n = 3. The band in western blotting was normalized to GAPDH

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