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Fig. 2 | Cancer Cell International

Fig. 2

From: SH2B1 promotes NSCLC cell proliferation through PI3K/Akt/mTOR signaling cascade

Fig. 2

SH2B1 promotes NSCLC cell proliferation in vitro. Cell Counting Kit-8 (CCK8) and soft agar assays (number and mean size of clonogenic colonies estimated after 2 weeks) were performed to analyze A549 and H1299 cells proliferation. a SH2B1-knockdown suppresses the proliferation of A549 cells. b SH2B1 overexpression elevates the H1299 cells proliferation. *P < 0.05, n = 3. c Soft agar colony formation assay of SH2B1-knockdown and control A549 cells in a 24-well dish (5 × 102 cells per well) for 2 weeks (n = 3). Representative images (left) and quantification of number of colonies (P = 0.0322, right upper) and average size of colony were shown (P = 0.0215, right lower). d Soft agar colony formation assay of SH2B1-overexpression and control H1299 cells in a 24-well dish (5 × 102 cells per well) for 2 weeks (n = 3). Representative images (left) and quantification of number of colonies (P = 0.0279, right upper) and average size of colony were shown (P = 0.0092, right lower). Data were mean ± SD, n = 3

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