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Fig. 4 | Cancer Cell International

Fig. 4

From: SH2B1 promotes NSCLC cell proliferation through PI3K/Akt/mTOR signaling cascade

Fig. 4

SH2B1 regulates NSCLC cell proliferation through Akt/mTOR pathway in vitro. a, b The effect of SH2B1 on Akt/mTOR signaling. a The protein levels of SH2B1, p-Akt, p-mTOR and PTEN in different panels of A549 and H1299 cells were assayed by Western blotting. b The protein levels of Akt/mTOR pathway targets (p-Akt, p-mTOR and PTEN) in different panels of H1299, which were subjected or not subjected to a Akt inhibitor, 10 μm/l deguelin for 24H, were assayed by Western blotting. Experiments were repeated three times and representative pictures are shown for a and b. GAPDH is used as an internal control. c CCK8 assay indicated that deguelin impaired the effect of SH2B1 on cell proliferation. Results are presented as mean ± SD

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