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Fig. 5 | Cancer Cell International

Fig. 5

From: Membrane expression of thymidine kinase 1 and potential clinical relevance in lung, breast, and colorectal malignancies

Fig. 5

Immunohistochemistry analysis of TK1 expression in lung cancer tissue. Lung cancer tissue arrays were stained with anti-TK1 antibody ab91651, GAPDH, or isotype antibody. Tissues were imaged in a light microscope at 20×. Analysis was conducted using a gray scale. The lower the gray value, the darker the staining. a Quantitative analysis of lung cancer IHC staining. The top graph shows that there is a statistically significant expression of TK1 in ~ 53% of the lung adenocarcinoma tissues and in ~ 58% of the lung squamous cell carcinoma tissues. The bottom graph shows the TK1 expression next to GAPDH and isotype controls. Background levels show no statistical difference between malignant and normal healthy tissues. Malignant and normal healthy tissue showed non-statistical difference in GAPDH expression, whereas TK1 expression did show a statically significant difference between TK1+ and TK1− tissues. b Images showing lung adenocarcinoma tissue positive and weakly positive for TK1, which we classified as TK1− with gray value quantification. The yellow circle in adenocarcinoma TK1+ image corresponds to lung papillary adenocarcinoma formed by abnormal proliferation of glanduli form structures of papillary disposition. The adenocarcinoma TK1− image shows pulmonary adenocarcinoma tissue with acinar pattern conformed by cells of convoluted nuclei, irregular membrane, and prominent central macronucleoli. The yellow arrows correspond to a diffuse weak positive nuclear staining for TK1. c Images showing lung squamous cell carcinoma tissue positive and weakly positive for TK1, which we classified as TK1− with gray value quantification. In the squamous cell carcinoma TK1+ image, the green line shows strong diffuse positive nuclear staining for TK1. The green arrow shows cytoplasmic background where there is evidence of infiltration in the underlying stroma shown by positive immunostaining against TK1. In the squamous cell carcinoma TK1− image, the red circle and arrows show weakly positive nuclear focal staining in poorly differentiated lung squamous carcinoma with solid pattern. ***P ≤ 0.001; ns = P > 0.05

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