Fig. 2From: Assessment of intratumor immune-microenvironment in colorectal cancers with extranodal extension of nodal metastasesa Mutational profiling of the 15 cases profiled through a hotspot multigene mutational custom panel, including 164 hotspot regions of AKT1, APC, BRAF, CTNNB1, KIT, KRAS, NRAS, PDGFRA, PIK3CA, PTEN and TP53 genes. In red are the samples showing a different mutational landscape in comparison to the other matched samples. (scale bars = 100 µm). b Representative Sequenom MassArray output profiles for KRAS c.436G > A (p.A146T), PIK3CA c.1633G > A (p.A545K), BRAF c1799T > A (p.V600E) and NRAS c.35G > A (p.G12D) mutations. On the right the correspondent Sanger chromatogramBack to article page