Fig. 2

BC094916 overexpression reduced cell proliferation and promoted cell apoptosis. a BC094916 overexpression suppressed the proliferation of SP 2/0 cells. The stable BC094916- and EGFP-expressing SP 2/0 cells (BC094916) and only EGFP-expressing SP 2/0 cells (vector) were cultured in vitro for 0, 1, 2 days. CCK8 assay was used to determine the cell proliferation. b, c BC094916 overexpression did not affect the cell cycle of SP 2/0 cells. The stable BC094916- and EGFP-expressing SP 2/0 cells (BC094916) and only EGFP-expressing SP 2/0 cells (vector) were cultured in vitro for 1 and 2 days. Cells were collected, stained with propidium solution (PI) and analyzed by flow cytometry (FACS). FACS profile on day 1 and 2 (b) and the statistical analysis on day 3 (c) of cell cycle were shown. d, e BC094916 overexpression up-regulated the apoptosis in SP 2/0 cells. The stable BC094916- and EGFP-expressing SP 2/0 cells (BC094916) and only EGFP-expressing SP 2/0 cells (vector) were cultured in vitro for 2 days. Cells were collected, stained with Annexin V-APC and propidium solution (PI) and analyzed by flow cytometry (FACS). Representative FACS profiles (d) and the statistical analysis (e) of the percentages of apoptic cells were shown. a–e The data are representative of three independent experiments. e Two-tailed student’s t test and. a, c Two-Way ANOVA were followed by Bonferroni post-tests to compare each column to control column. Error bars, SEM. **p < 0.01, ****p < 0.0001