Fig. 6From: Identification of critical genes to predict recurrence and death in colon cancer: integrating gene expression and bioinformatics analysisKnock-down of VCAN inhibits HCT-116 cell colony formation and migration (a, b) small interfering RNA (siRNA)-mediated knockdown ofVCAN. HCT116 cells were transfected with negative control siRNA (NC) and siRNA against VCAN (si-VCAN). After transfection, Expression of VCAN was determined by qRT-PCR and immunoblot analysis. HCT116 cells were transiently transfected with negative control siRNA and siRNA against VCAN, and then subjected to (c, d) transwell migration assay, and e colony formation, respectively. **p < 0.01Back to article page