Fig. 2From: Marsdenia tenacissima extract induces apoptosis and suppresses autophagy through ERK activation in lung cancer cellsMTE treatment induced apoptosis in NSCLC cells. A549 and H1975 cells were treated with 0, 20, 40, 60 and 80 mg/ml MTE for 24 h. a Apoptotic cells were counted by Annexin V/PI assay. In the four fields of the original images, the dots indicated the number of Annexin V−/PI− (bottom-left field indicates live cells), Annexin V+/PI− (bottom-right field indicates early apoptotic cells), Annexin V+/PI+ (top-right field indicates late apoptotic cells), and Annexin V−/PI+ cells (top-left field indicates dead cells), respectively. b The percentage of early and late apoptotic cells were quantified, respectively. Early-stage apoptotic cells in the figures were shown in red, and late-stage apoptotic cells were shown in blue. c The protein levels of Caspase 3 and PARP were determined by Western blot, and the expression ratio was counted. β-Actin was used as loading control. Data are one representative experiment performed in triplicate. d The Caspase 3 activities in MTE treated cells were detected. A549 cells were shown in red, and H1975 cells were shown in blue. *P < 0.05, **P < 0.01 vs control groupBack to article page