Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: Marsdenia tenacissima extract induces apoptosis and suppresses autophagy through ERK activation in lung cancer cells

Fig. 3

MTE treatment disrupted autophagic flux in NSCLC cells. a, c The protein levels of LC3-II and p62 in treated cells were determined by Western blot, and the ratio of protein levels was counted. a Cells were treated with MTE at the concentration of 0, 10, 20, 40, 60 and 80 mg/ml, respectively. b Cells were treated with MTE at 0, 4, 8, 12 and 24 h, respectively. c Cells were pretreated with 40 mg/ml MTE for 24 h following by Baf A1 (5 nM) or EBSS for another 4 h. β-Actin was used as loading control. d Endogenous LC3 levels (green fluorescence) were visualized on confocal microscope by immunofluorescence staining. e LC3 dots in A549 and H1975 cells were counted. Data represents mean ± SEM of at least 100 cells scored. *P < 0.05 for compared two groups

Back to article page