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Fig. 1 | Cancer Cell International

Fig. 1

From: R-spodin2 enhances canonical Wnt signaling to maintain the stemness of glioblastoma cells

Fig. 1

Expression pattern and potentiation effect of RSPOs in U251 cells. a mRNA expression levels of RSPO2/3 and their receptors LGR4–6 were determined in U87 and U251 cells. U251 cells were pre-treated in serum-free medium for 24 h, then cultured in serum-free medium containing different Wnt ligands (20 ng/ml Wnt3A, Rspo2, Rspo3) for another 24 h. Rspo2 shows prominent potentiation effect on Wnt3A-induced canonical β-catenin downstream targets (b), and RSPO-LGR genes (c). Blk indicates U251 cells cultured in serum-free medium containing 0.1% DMSO. d 7TGP-transduced U251 cells were pre-treated in serum-free medium for 24 h, then cultured in serum-free medium containing different Wnt stimuli for another 24–48 h. Representative Western Blot showing the protein expression levels of canonical β-catenin downstream targets in 7TGP-transduced U251 cells with Wnt ligand treatment

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