Fig. 5

Impact of afadin silencing on the metastasis phenotype of U2OS cells. a Western blotting was used to examine the effects of silencing afadin and the activation of the Ras/Raf/MEK/ERK signaling pathway in the U2OS cell line. b The corresponding statistical analysis of the activation of the Ras/Raf/MEK/ERK signaling pathway. c The wound healing assay was utilized to detect the migration ability of the U2OS cell line in vitro. d The transwell chamber method was utilized to detect the impact of afadin silencing on the invasive ability of cells in vitro. e The corresponding statistical analysis of invaded cells. f Membrane expressions of afadin in OS tissues and normal bone tissues. **P < 0.01 compared with the scramble group