Fig. 5

Released HtrA2/Omi triggers an activation of caspase-9-related cellular apoptosis. a, b The mitochondrial potential was determined using a JC-1 kit in PANC-1 cells. Mdivi-1 was used to inhibit mitochondrial fragmentation. Furthermore, two independent siRNAs against HtrA2/Omi were transfected into PANC-1 cells to suppress HtrA2/Omi expression. The red-to-green ratio was recorded to quantify the mitochondrial potential. c The activation of caspase-9 was measured using an ELISA to evaluate the activity of caspase-9. Mdivi-1 was used to inhibit mitochondrial fragmentation. Furthermore, two independent siRNAs against HtrA2/Omi were transfected into PANC-1 cells to suppress HtrA2/Omi expression. d, e Expression of caspase-9 was determined via immunofluorescence. f–j Western blotting was performed to detect alterations in proapoptotic proteins and antiapoptotic factors. Mdivi-1 was used to inhibit mitochondrial fragmentation. Additionally, two siRNAs against HtrA2/Omi were transfected into PANC-1 cells to suppress HtrA2/Omi expression. *p < 0.05