Fig. 3
From: Genome editing of oncogenes with ZFNs and TALENs: caveats in nuclease design
Expression analysis of E6 in CaSki cells showed no significant difference between control and treated groups. a RT-PCR data indicated that although full length E6 levels were decreased in treated, E6* spliced variant (300 bp) levels remained the same in both control and treated. This was further validated by immunocytochemical analysis for E6 proteins (b–g). ZFN treated CaSki cells showed no difference in E6 (red) levels after editing (e–g) when compared to control cells (b–d). Western blot analysis corroborated with the immunocytochemical analysis indicating no significant difference in E6 levels before and after treatment (h). Magnification ×600