Fig. 2

Overexpression of SKA3 promotes HeLa and SiHa cell proliferation, clone formation and migration. a HeLa and SiHa cells with stable SKA3 overexpression, SKA3 knockdown and control plasmid expression were generated and confirmed by western blotting. b Proliferation of HeLa and SiHa cells with stable SKA3 overexpression, SKA3 knockdown and control plasmid expression was assessed using a CCK-8 assay. c Clone formation assay in HeLa and SiHa cells with stable SKA3 overexpression, SKA3 knockdown and control plasmid expression. The migration of HeLa and SiHa cells was examined by d a Transwell assay and e a wound-healing assay. f Representative cell cycle data were measured by flow cytometry in HeLa and SiHa cells with stable SKA3 overexpression, SKA3 knockdown and control plasmid. All data (mean ± SD; n = 3) were analyzed by Student’s t test (*p < 0.05, **p < 0.01, over-SKA3 vs. control vector. ^#p < 0.05, ^##p < 0.01, SKA3 knockdown vs. control vector)