Fig. 6

An inhibitor of Akt blocked the cell proliferation abilities induced by SKA3 overexpression in HeLa cells. a The proliferation curves for HeLa cells with control vector expression, SKA3 overexpression, vector expression + inhibitor treatment and SKA3 overexpression + inhibitor treatment according to a CCK8 assay. b Representative images of clone formation in HeLa cells with control vector expression, SKA3 overexpression, vector expression + inhibitor treatment and SKA3 overexpression + inhibitor treatment. c Clone formation assay in HeLa cells with control vector expression, SKA3 overexpression, vector expression + inhibitor treatment and SKA3 overexpression + inhibitor treatment. d Representative cell cycle data were measured by flow cytometry in HeLa cells with control vector expression, SKA3 overexpression and SKA3 overexpression + inhibitor treatment. e Representative western blot analyses of proteins related to the PI3K–Akt pathway and the cell cycle in HeLa cells with control vector expression, SKA3 overexpression and SKA3 overexpression + inhibitor treatment. f Activity of E2F1 in HeLa cells with control vector expression, SKA3 overexpression and SKA3 overexpression + inhibitor treatment