Fig. 5
IL-2 enhanced sorafenib-initiated mitochondrial fission.a–f Western blotting was used to analyse the proteins related to mitochondrial fusion and mitochondrial fission. Drp1, Fis1 and Mff are factors involved in mitochondrial fission. In contrast, mitochondrial fusion is regulated by Mfn1 and Opa1. IL-2 and sorafenib co-treatment elevated the mitochondrial fission proteins and repressed the mitochondrial fusion factors. g, h Mitochondrial fission was observed via immunofluorescence using the Tom20 antibody. Then, the average length of mitochondria was measured in HepG2 cells. *P < 0.05 vs. control group; #P < 0.05 vs. sorafenib group.Cont control