Fig. 7

TEAD4 is required for TGF-β1-induced EMT in HNSCC cells. a The abundance of EMT-related markers E-cadherin, N-cadherin, vimentin and snail were measured by western blot (WB) in the Cal27 and Fadu cells following TEAD4 knockdown. b The mRNA and protein abundance of TEAD4 was measured by real-time RT-PCR and western blot when cells were treated with human recombinant TGF-β1 (10 ng/ml) at indicated time points. c The abundance of EMT-related markers E-cadherin, N-cadherin, vimentin and Snail were measured by immunofluorescence (c) and western blot assays (d) when Cal27 cells were treated siTEAD4 or in combination with TGF-β1 (10 ng/ml) for 48 h. e Cell migration was measured via wound healing assay when Cal27 cells were treated siTEAD4 or in combination with TGF-β1 (10 ng/ml) for 48 h. Scale bar: 100 μm. f Correlation between generic EMT score for 502 HNSCC samples from TCGA dataset and TEAD4 expression. Generic EMT score was calculated following the method of single-sample Gene Set Enrichment Analysis (ssGSEA) [36]. The correlation coefficient (R) and P-value were based on Pearson’s product-moment correlation analysis; R = 0.15, P = 0.0008. *P < 0.05, **P < 0.01, ANOVA analyses