Fig. 2

RelB-silencing inhibits the DLD-1 cell growth. a The cell growth rates were monitored by a real-time x-Celligence system for 72 h. b Cell proliferation was detected by CCK-8 assay. OD450 was measured using spectrophotometer microplate reader at 24, 48, and 72 h. c Cell proliferation was detected by Brdu assay. OD450 was measured after transfection with siRNA for 24 h. d Cell proliferation was evaluated by colony formation assay. 1000 cells were seeded in 6-cm dishes and cultured for 2 weeks. Colonies containing > 50 cells were counted. e Cell apoptosis assay was examined by Flow cytometry at 0, 24, 48, and 72 h. f Western blotting analysis of the protein expression of total AKT, p-AKT^Ser473, p-AKT^Ser308, p-mTOR^Ser2448, p-p70S6K^Thr389, PTEN, and p-GSK-3β^Ser9 in siRelB and sictrl cells. Protein levels were normalized against Actin. *p < 0.05; **p < 0.01; ***p < 0.001