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Fig. 4 | Cancer Cell International

Fig. 4

From: RelB plays an oncogenic role and conveys chemo-resistance to DLD-1 colon cancer cells

Fig. 4

RelB-silencing hampers the migration and invasion abilities of DLD-1 cells. a The migration abilities of the two established cells were assessed by a real-timex-Celligence system for 24 h. b Representative images and data of a Transwell migration assay. The number of migratory cells were counted and photographed at 24 h. c Cell migration ability assessed by wound healing assay at 0, 24, 48, and 72 h. d The invasion abilities of the two established cells were detected by a real-time x-Celligence system using CIM-plate pre-coated with Matrigel (1:40) and Cell invasion was continuous monitored for 24 h. e Representative images and data of a Transwell invasion assay. Transwell chambers were pre-coated with Matrigel (1:8) and the number of invasive cells were counted and photographed at 24 h. f Western blotting analysis of the protein expression of MMP9, MMP2, and ITGB1 in siRelB and sictrl cells. Protein levels were normalized against Actin.*p < 0.05; **p < 0.01; ***p < 0.001

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