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Fig. 5 | Cancer Cell International

Fig. 5

From: Mifepristone inhibits non-small cell lung carcinoma cellular escape from DNA damaging cisplatin

Fig. 5

Clonogenic capacity of NSCLC cells following second-line chemotherapy. a, b The treatment schedule for A549 and H23 cells, respectively. Twelve days after first-line treatment (A459 cells) or 25 days after first-line treatment (H23 cells), a second-line treatment was given. At the end of the experiment (day 18 for A549 cells or day 40 for H23 cells), 500 viable cells taken upon second-line treatment with vehicle (indicated as ‘first-line only’ in right panels), CDDP, 20 μM MF, or CDDP plus 20 μM MF were seeded and provided untreated media to assess their clonogenic survival. Cells were allowed to proliferate for 7 days, at which time media was aspirated, cells were washed with PBS, fixed with methanol and stained with crystal violate. Colonies having > 30 cells were considered positive and consequently manually counted. Results were analyzed using one-way ANOVA followed by Tukey’s Multiple Comparison Test. *Indicates p < 0.05 and **indicates p < 0.01 compared against ‘first-line only’ groups. For A549 cells, CDDP was used at a 100 μM concentration. For H23, CDDP was used at a 40 μM concentration

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