Fig. 5

MiR-133b suppressed migration, invasion and EMT process of ESCC cells via regulating EGFR. a The migration ability of KYSE150 and ECa109 cells was evaluated by scratch assay. b The migration rate was calculated and shown. c The invasion ability of KYSE150 and ECa109 cells was detected by transwell assay. d The number of invasive cells was counted. e The protein levels of Fibronectin, Vimentin, N-cadherin, E-cadherin, MMP-2, and MMP-9 in KYSE150 and ECa109 cells were assessed by western blotting. The experimental data were representative of three independent experiments. Results were expressed as mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001