Fig. 4From: Increased activated regulatory T cell subsets and aging Treg-like cells in multiple myeloma and monoclonal gammopathy of undetermined significance: a case control studyIn vitro suppressive function assay from bone marrow of newly diagnosed MM patient. Representative curves are shown for a MM patient. a Negative control: unlabeled CD4+ CD25− T cells alone. b Positive control 1: CFSE-labeled CD4+ CD25− T cells without anti-CD3/CD28 beads. c Positive control 2: CFSE-labeled CD4+ CD25− T cells with anti-CD3/CD28 beads. d aTreg group: CFSE-labeled CD4+ CD25− T cells cocultured with aTreg at a ratio of 1:1. e rTreg group: CFSE-labeled CD4+ CD25− T cells cocultured with rTreg at a ratio of 1:1. f non-Treg: CFSE-labeled CD4+ CD25− T cells cocultured with non-Treg at a ratio of 1:1Back to article page