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Fig. 4 | Cancer Cell International

Fig. 4

From: Increased activated regulatory T cell subsets and aging Treg-like cells in multiple myeloma and monoclonal gammopathy of undetermined significance: a case control study

Fig. 4

In vitro suppressive function assay from bone marrow of newly diagnosed MM patient. Representative curves are shown for a MM patient. a Negative control: unlabeled CD4+ CD25 T cells alone. b Positive control 1: CFSE-labeled CD4+ CD25 T cells without anti-CD3/CD28 beads. c Positive control 2: CFSE-labeled CD4+ CD25 T cells with anti-CD3/CD28 beads. d aTreg group: CFSE-labeled CD4+ CD25 T cells cocultured with aTreg at a ratio of 1:1. e rTreg group: CFSE-labeled CD4+ CD25 T cells cocultured with rTreg at a ratio of 1:1. f non-Treg: CFSE-labeled CD4+ CD25 T cells cocultured with non-Treg at a ratio of 1:1

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