Fig. 2

EE-15-one induces rapid cell detachment by inhibiting cell–substrate adhesion. a MCF-7, MDA-MB-231, and BT-20 cells exposed to 5 µM EE-15-one for 24 h were imaged by light microscopy using a 10× magnification objective. Scale bar, 10 µm. b Cell rounding of MCF-7 cells exposed to 5 µM EE-15-one, 0.5 µM ESE-15-one or DMSO (vehicle control) was quantified by counting the spread and rounded cells in at least 4 fields per condition in three independent experiments. c Cell rounding of MDA-MB-231 cells exposed to 5 µM EE-15-one, or DMSO (vehicle control) was quantified by counting the spread and rounded cells in at least 4 fields per condition in three independent experiments. The average of the three independent experiments was plotted while error bars represent the s.e.m. *P < 0.05 in a Student’s t-test