Fig. 3

EE-15-one exposure leads to inhibition of cell–substrate adhesion. The effect of EE-15-one on cell–substrate adhesion was quantified by plating suspended MCF-7 cells onto tissue culture treated plates and calculating the number of adhered cells at different times. Suspended cells were either treated with DMSO as control for 2 h, with 5 μM EE-15-one for 2 h or with 5 μM EE-15-one at the time of plating. The average of at least three biologically independent experiments have been averaged with error bars representing s.e.m. *Indicates statistically significant decreases in adhesion (P < 0.05, student’s t-test between DMSO and treated)