Fig. 2

HVJ-E induced mitochondrial apoptosis in HeLa cells in a caspase-dependent manner. HeLa cells were infected with HVJ-E at the indicated MOI for 24 h. a Expression levels of cleaved caspase-3, cleaved caspase-9, cleaved PARP, BAX, and Bcl-2 were detected by Western blot. β-Actin was used as a control. b Gray value analysis of Bcl-2, Bax, and the ratio of Bcl-2 to Bax. e–g Cells were treated with the pan-caspase inhibitor, Z-VAD-FMK (50 μM), prior to infection with HVJ-E at 800 MOI. Cleaved caspase-3 and PARP were detected by Western blot. Densities of protein bands were analyzed and normalized to β-actin. c Cells were treated with Z-VAD-FMK prior to infection. Cellular apoptosis was assessed and analyzed by flow cytometry. *P < 0.05, **P < 0.01